文章：

胃粘膜相关淋巴组织淋巴瘤细胞中幽门螺杆菌CagA蛋白的检测：临床和生物学意义

Detection of the Helicobacter pylori CagA protein in gastric mucosa-associated lymphoid tissue lymphoma cells: clinical and biological significance

原文发布日期：2013-07-12

DOI: 10.1038/bcj.2013.22

类型: Original Article

开放获取: 是

英文摘要：

We previously reported that CagA can be translocated into B cells in Helicobacter pylori (HP) coculture media, and the translocation appears biologically significant as activation of the relevant cellular pathways was noticed. In this study, we further explore if CagA can be detected in malignant B cells of HP-positive gastric mucosa-associated lymphoid tissue (MALT) lymphoma. Expression of CagA was evaluated by immunohistochemistry. CagA expression was further confirmed by western blot analysis. The association between CagA expression in malignant B cells and tumor response to HP eradication therapy (HPE) was evaluated in 64 stage IE gastric MALT lymphoma patients. We detected CagA expression in 31 (48.4%) of 64 patients: 26 (68.4%) of the 38 HP-dependent cases and 5 (19.2%) of the 26 HP-independent cases (P<0.001). Patients with CagA expression responded to HPE quicker than those without (median time to complete remission, 3.0 vs 6.5 months, P=0.025). Our results indicated that CagA can be translocated into malignant B cells of MALT lymphoma, and the translocation is clinically and biologically significant.

摘要翻译： 

我们先前报道过，在幽门螺杆菌共培养介质中，CagA可转运至B细胞内，且这种转运具有生物学意义，因为观察到相关细胞通路的激活。本研究进一步探讨在HP阳性胃黏膜相关淋巴组织淋巴瘤的恶性B细胞中是否能检测到CagA。通过免疫组织化学法评估CagA表达，并经蛋白质印迹分析进一步确认。在64例IE期胃MALT淋巴瘤患者中，评估了恶性B细胞CagA表达与肿瘤对HP根除治疗反应之间的关联。在64例患者中有31例（48.4%）检测到CagA表达：38例HP依赖性病例中的26例（68.4%）及26例HP非依赖性病例中的5例（19.2%）（P<0.001）。表达CagA的患者对HPE的反应较未表达者更快（完全缓解中位时间：3.0个月 vs 6.5个月，P=0.025）。我们的结果表明CagA可转运至MALT淋巴瘤的恶性B细胞中，且这种转运具有临床与生物学意义。

原文链接：

Detection of the Helicobacter pylori CagA protein in gastric mucosa-associated lymphoid tissue lymphoma cells: clinical and biological significance