文章：

通过流式细胞术细胞分选和基因表达谱比较早期CD105阳性红细胞与CD34阳性普通前体细胞的分子通路

Molecular pathways of early CD105-positive erythroid cells as compared with CD34-positive common precursor cells by flow cytometric cell-sorting and gene expression profiling

原文发布日期：2013-01-11

DOI: 10.1038/bcj.2012.45

类型: Original Article

开放获取: 是

英文摘要：

Special attention has recently been drawn to the molecular network of different genes that are responsible for the development of erythroid cells. The aim of the present study was to establish in detail the immunophenotype of early erythroid cells and to compare the gene expression profile of freshly isolated early erythroid precursors with that of the CD34-positive (CD34+) compartment. Multiparameter flow cytometric analyses of human bone marrow mononuclear cell fractions (n=20) defined three distinct early erythroid stages. The gene expression profile of sorted early erythroid cells was analyzed by Affymetrix array technology. For 4524 genes, a differential regulation was found in CD105-positive erythroid cells as compared with the CD34+ progenitor compartment (2362 upregulated genes). A highly significant difference was observed in the expression level of genes involved in transcription, heme synthesis, iron and mitochondrial metabolism and transforming growth factor-β signaling. A comparison with recently published data showed over 1000 genes that as yet have not been reported to be upregulated in the early erythroid lineage. The gene expression level within distinct pathways could be illustrated directly by applying the Ingenuity software program. The results of gene expression analyses can be seen at the Gene Expression Omnibus repository.

摘要翻译： 

近期研究特别关注调控红系细胞发育的基因分子网络。本研究旨在详细阐明早期红系细胞的免疫表型，并将新鲜分离的早期红系前体细胞与CD34阳性（CD34+）细胞群的基因表达谱进行比对。通过多参数流式细胞术对人骨髓单个核细胞组分（n=20）进行分析，确定了三个不同的早期红系阶段。采用Affymetrix芯片技术对分选的早期红系细胞进行基因表达谱分析，发现CD105阳性红系细胞与CD34+祖细胞群相比有4524个基因存在差异调控（其中2362个基因上调）。在涉及转录、血红素合成、铁及线粒体代谢、转化生长因子-β信号传导等通路的基因表达水平上观察到显著差异。与近期发表数据对比显示，超过1000个基因此前未在早期红系谱系中报道过上调表达。应用Ingenuity软件可直接展示特定通路内的基因表达水平，基因表达分析结果可见于Gene Expression Omnibus数据库。

原文链接：

Molecular pathways of early CD105-positive erythroid cells as compared with CD34-positive common precursor cells by flow cytometric cell-sorting and gene expression profiling