文章：

AL淀粉样变性患者睾丸癌抗原表达及免疫原性

Cancer-testis antigen expression and immunogenicity in AL amyloidosis

原文发布日期：2012-09-14

DOI: 10.1038/bcj.2012.32

类型: Original Article

开放获取: 是

英文摘要：

Light-chain amyloidosis (AL) is a plasma cell dyscrasia closely related to multiple myeloma. In multiple myeloma, the cancer-testis antigens (CTAs) CT7 (MAGE-C1), CT10 (MAGE-C2) and MAGE-A CTAs are expressed in up to 80% of cases. In this study, we investigated the expression and immunogenicity of several CTAs in patients with AL amyloidosis in a total of 38 bone marrow specimens by employing standard immunohistochemistry techniques on paraffin-embedded archival tissues. Plasma samples from 35 patients (27 with matched bone marrow samples) were also analyzed by ELISA for sero reactivity to a group of full-length CTA proteins. CT7 was present in 25/38 (66%) while CT10 was demonstrated in 3/38 and GAGE in 1/38 AL amyloid cases. The expression pattern was mostly focal. There were no significant differences with regard to organ involvement, response to treatment, or prognosis in CTA positive compared to negative cases. None of the specimens showed spontaneous humoral immunity to CT7, but sero reactivity was observed in individual patients to other CTAs. This study identifies CT7 as the prevalent CTA in plasma cells of patients with AL amyloidosis. Further analyses determining the biology of CTAs in AL amyloidosis and their value as potential targets for immunotherapy are warranted.

摘要翻译： 

轻链淀粉样变性（AL）是一种与多发性骨髓瘤密切相关的浆细胞恶液质。在多发性骨髓瘤中，癌症-睾丸抗原（CTAs）CT7（MAGE-C1）、CT10（MAGE-C2）及MAGE-A CTAs的表达率高达80%。本研究通过石蜡包埋存档组织的标准免疫组化技术，对38例AL淀粉样变性患者的骨髓标本中多种CTAs的表达情况及免疫原性进行了分析。同时采用ELISA法检测35例患者（其中27例具有配对骨髓样本）血浆对一组全长CTA蛋白的血清反应性。结果显示，CT7在25/38（66%）的AL淀粉样变性病例中表达，CT10在3/38病例中表达，GAGE在1/38病例中表达，表达模式多呈局灶性。CTA阳性组与阴性组在器官受累、治疗反应或预后方面均无显著差异。所有标本均未显示对CT7的自发性体液免疫，但在个别患者中观察到对其他CTAs的血清反应性。本研究确认CT7是AL淀粉样变性患者浆细胞中最主要的CTA。有必要进一步分析CTAs在AL淀粉样变性中的生物学特性及其作为免疫治疗潜在靶点的价值。

原文链接：

Cancer-testis antigen expression and immunogenicity in AL amyloidosis