文章：

B细胞淋巴瘤中的MicroRNA特征

MicroRNA signatures in B-cell lymphomas

原文发布日期：2012-02-17

DOI: 10.1038/bcj.2012.1

类型: Original Article

开放获取: 是

英文摘要：

Accurate lymphoma diagnosis, prognosis and therapy still require additional markers. We explore the potential relevance of microRNA (miRNA) expression in a large series that included all major B-cell non-Hodgkin lymphoma (NHL) types. The data generated were also used to identify miRNAs differentially expressed in Burkitt lymphoma (BL) and diffuse large B-cell lymphoma (DLBCL) samples. A series of 147 NHL samples and 15 controls were hybridized on a human miRNA one-color platform containing probes for 470 human miRNAs. Each lymphoma type was compared against the entire set of NHLs. BL was also directly compared with DLBCL, and 43 preselected miRNAs were analyzed in a new series of routinely processed samples of 28 BLs and 43 DLBCLs using quantitative reverse transcription-polymerase chain reaction. A signature of 128 miRNAs enabled the characterization of lymphoma neoplasms, reflecting the lymphoma type, cell of origin and/or discrete oncogene alterations. Comparative analysis of BL and DLBCL yielded 19 differentially expressed miRNAs, which were confirmed in a second confirmation series of 71 paraffin-embedded samples. The set of differentially expressed miRNAs found here expands the range of potential diagnostic markers for lymphoma diagnosis, especially when differential diagnosis of BL and DLBCL is required.

摘要翻译： 

准确的淋巴瘤诊断、预后和治疗仍需额外的标志物。我们在一个涵盖所有主要B细胞非霍奇金淋巴瘤（NHL）类型的大型系列中，探讨了微小RNA（miRNA）表达的潜在意义。所得数据还用于识别伯基特淋巴瘤（BL）与弥漫大B细胞淋巴瘤（DLBCL）样本中差异表达的miRNA。将147例NHL样本和15例对照样本在含470条人类miRNA探针的单色人miRNA平台上进行杂交。每种淋巴瘤类型均与整个NHL系列进行比较；BL还与DLBCL直接比较。随后，利用定量逆转录-聚合酶链反应，在28例BL和43例DLBCL的常规处理新系列中对43条预选miRNA进行分析。128条miRNA的签名可表征淋巴瘤肿瘤，反映淋巴瘤类型、细胞来源和/或特定癌基因改变。BL与DLBCL的比较分析获得19条差异表达miRNA，并在第二组71例石蜡包埋样本中得到验证。本研究发现的差异表达miRNA集合扩展了淋巴瘤诊断的潜在诊断标志物范围，尤其在需要鉴别BL与DLBCL时。

原文链接：

MicroRNA signatures in B-cell lymphomas